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fluidigm 201063 cytof cytometry  (fluidigm)


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    fluidigm fluidigm 201063 cytof cytometry
    Fluidigm 201063 Cytof Cytometry, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluidigm+201063+cytof+cytometry/Maxpar+Nuclear+Antigen+Staining+Buffer+Set/pmc10436398__40779_2023_474_MOESM1_ESM-154-299-299
    Average 95 stars, based on 86 article reviews
    fluidigm 201063 cytof cytometry - by Bioz Stars, 2026-09
    95/100 stars

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    Over Expression:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Microarray:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Staining:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Fluorescence:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Microscopy:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Transfection:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Blocking Assay:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Cytometry:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..

    Activation Assay:

    Article Title:
    Article Snippet: .. OE overexpression, SQLE squalene epoxidase Table S1 A list of the antibodies and reagents used in this research Reagent or resource Source Identifier TCGA dataset Xena LUNG cohort GSE135222 GEO GSE135222 GSE126044 GEO GSE126044 SQLE antibody ProteinTech 12544-1-AP HMGCR antibody ProteinTech 13533-1-AP CD8 antibody GeneTech GT2280 CD8 antibody Abcam ab217344 CD86 antibody Abcam ab220188 F4/80 antibody Abcam ab6640 Goat anti-rabbit IgG H&L (Alexa Fluor 488) Abcam ab150077 Goat anti-rat IgG H&L (Alexa Fluor 594) Abcam ab150160 Goat anti-mouse IgG H&L (Alexa Fluor 488) Abcam ab150113 Goat anti-rabbit IgG H&L (Alexa Fluor 594) Abcam ab150080 SQLE overexpression lentivirus Genechem N/A The tissue microarray Outdo BioTech HLugA150CS01 The NSCLC samples In-house cohort N/A DAB Proteintech PR30010 Hematoxylin staining solution Beyotime C0107 Fluorescence microscope Echo Revolve Mouse lung cancer Lewis cell line KeyGEN KG070 Lentiviral transfection experiments GenePharma Co., Ltd. N/A C57BL/6 mice SLAC Co., Ltd. N/A Voluntary running wheels Yuyan instruments 1085 DAPI Invitrogen D1306 Palladium ions Fluidigm N/A Isothiocyanobenzyl-EDTA Lumiprobe M030 Fc receptor blocking reagent BioLegend 422302 Helios mass cytometer Fluidigm Helios CyTOF CD4 Fluidigm 3145002C CD45 Fluidigm 3089005C CD3e Fluidigm 3152004C CD86 Fluidigm 3172016C F4/80 Fluidigm 3146008C FoxP3 Fluidigm 3158003C CD11b (Mac-1) Fluidigm 3209003C Ly-6G/C (Gr-1) Fluidigm 3141005C CD206 (MMR) Fluidigm 3169021C CD27 Fluidigm 3150017C CD25 (IL-2R) Fluidigm 3151007C CD279 (PD-1) Fluidigm 3159006C CD274 (PD-L1) Fluidigm 3153016C CD161 (NK1.1) Fluidigm 3170002C CD152 (CTLA-4) Fluidigm 3154008C IFNg Fluidigm 3165003C CD223 (LAG-3) Fluidigm 3174019C CD14 Fluidigm 3156009C CD21 Fluidigm 3168010C TCRb Fluidigm 3143010C CD40 Fluidigm 3161020C Granzyme B Fluidigm 3171002C FITC Fluidigm 3160011C APC Fluidigm 3176007C Cisplatin Fluidigm 201064 Intercalator-Ir Fluidigm 201192A Maxpar cell staining buffer (500 ml) Fluidigm 201068 Maxpar fix and perm buffer (100 ml) Fluidigm 201067 Maxpar water (500 ml) Fluidigm 201069 Maxpar cell acquisition solution plus for CyTOF XT-1L Fluidigm 201244 Maxpar nuclear antigen staining buffer set Fluidigm 201063 CyTOF cytometry by time-of-flight, DAB diaminobenzidine, DAPI 4',6-diamidino-2-phenylindole dihydrochloride, HMGCR 3-hydroxy-3-methylglutaryl-coenzyme A reductase, NSCLC non-small cell lung cancer, SQLE squalene epoxidase, TCGA The Cancer Genome Atlas, FITC fluorescein isothiocyanate isomer I, APC allophycocyanin, IFNg interferon gamma, CTLA-4 cytotoxic T-lymphocyte associated protein 4, MMR macrophage mannose receptor, PD-1 programmed cell death protein 1, LAG-3 lymphocyte activation gene-3 ..



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